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Model Number : In-Ra1379
Place of Origin : CHINA
MOQ : 1 Box
Price : Negotiable
Payment Terms : L/C, Western Union, T/T
Supply Ability : 1000kits/week
Delivery Time : 5-8 working days
Packaging Details : 96wells/box or 48wells/box
Specification : 48wells or 96wells
Method : Sandwich method
Cat. No. : In-Ra1379
Purpose : For research use
Expiration date : 180 days
Storage temperature : 2-8℃
Brand Name : BIOVANTION
Rat Super Oxidase Dimutase ,SOD/Cell Surface Cu-only Superoxide Dismutase Elisa Kit
Materials provided with the kit
Materials provided with the kit | 48 determinations | 96 determinations | Storage | |
1 | User manual | 1 | 1 | R.T. |
2 | Closure plate membrane | 2 | 2 | R.T. |
3 | Sealed bags | 1 | 1 | R.T. |
4 | Microelisa stripplate | 1 | 1 | 2-8℃ |
5 | Standard:2700pg/ml | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
6 | Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
7 | HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
8 | Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
9 | Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
10 | Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
11 | Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
12 | Wash solution | 20ml (20X)×1bottle | 20ml (30X)×1bottle | 2-8℃ |
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to SOD. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for SOD. is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SOD. and HRP conjugated TNF-α antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SOD.. You can calculate the concentration of SOD. in the samples by comparing the OD of the samples to the standard curve.
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Rat Superoxide Dismutase Elisa Kit SOD Elisa Kit Cell Surface Cu-Only Images |